Proteins are encoded by genes and are one of the major constituents of the cell. Enzymatic proteins control the chemical reactions important for the functioning of the cell, other proteins participate in its structure and others, regulate its major functions, including proliferation. Proteins are the target of many drugs used to treat different pathologies. Their analysis is therefore essential to understand the cellular functioning and develop new therapeutic strategies. Until very recently, this analysis was difficult because of their huge diversity. Our project is to obtain an accurate cartography of all the proteins of the blood cell (the proteome) in an inherited pathology, sickle cell disease, and to follow its normalization after gene therapy.